Rat Podocytes are isolated from normal rat kidney. The cells are characterized by immunofluorescence with antibodies specific to podocin, Ang1, Nephrin, ACTN4, NPHS2. T25 flasks is required for cell adhension to the culture vessels. Grow cells in ECM-coated culture vessels with 5% CO2. Each vial contains at least 1x10^6 cells per ml.